BACTERIA TESTING

                                                Microbiology Department
                                                Brigham Young University
                                                775 WIDB
                                                P.O. Box 25253
                                                Provo, Utah 84602-5253

                                                                                                            June 18, 1999

TO: American Silver, LLC

From: David Revelli
Brigham Young University

        

 We have tested and compared the activity of American Silver's ASAP solution against three commercial colloidal silver solutions using the Minimum Inhibitory Concentration test (MIC) and the Minimum Bactericidal Concentration (MBC) test. The data obtained suggests that American Silver's ASAP solution is at least two to three times more effective depending on the bacteria tested than the commercial colloidal silver products tested.

 

David A. Revelli

Microbiologist

Brigham Young University

 

ASAP SILVER SOLUTION versus Antibiotics

                                    University Study:

To: American Silver, LLC (American Biotech Labs)               Microbiology Department
From: David Revelli                                                                  Brigham Young University
Brigham Young University                                                      775 WIDB
October 15, 1999                                                                      P.O. Box 25253
                                                                                                  Provo, Utah 84602-5253

 

This page contains data from seven strains of potentially pathogenic bacteria. As the data suggests, the ASAP Silver Solution has a greater ability on average to kill the bacteria tested than four of the five antibiotics to which it was compared. Each antibiotic has its own niche to fill, but not one of the antibiotics tested work equally well on every bacterial strain that it was tested against, ASAP Silver Solution had similar results for each bacteria - it was able to kill each bacteria tested. Furthermore, there are antibiotic-resistant strains that may or may not succumb to a given antibiotic. This is where the ASAP Silver Solution data comes in-although ASAP Silver Solution may not have inhibited a strain of bacteria at a lower concentration than a given antibiotic, it inhibited every strain of bacteria tested, which is more than can be suggested for some of the other antibiotics, given the data.

 

Minimum Inhibitory Concentration of Antibiotics from Five Different Classes versus ASAP Silver Solution: Performed by David A. Revelli, Brigham Young University

 

Bacteria Tested

Antibiotic  Tetra-
cycline

Antibiotic
 oflxacin

Antibiotic penicillin
G

Antibiotic cefapera-
zone

Antibiotic erythro-
mycin

ASAP Silver Solution

E.
coli B

1.67+-0.59/
Killed@
>5ppm
/3

0.104+-
0.037/
Killed@
0.130+-
0.037ppm
/
3

No Inh/
3
 

.625/
Killed@
.625ppm
/3

5/
Killed@
>5ppm
/3

2.5/
Killed@
2.5ppm
/3

E.
aerogenes

2.5/Killed@ >5ppm/3

0.078/
Killed@
0.104+-
0.037ppm
/3

No Inh/
3
 

2.92+-1.56/
Killed@
>5ppm
/3

No Inh/

2.5/
Killed@
2.5ppm
/3

E.
cloacae

1.67+-0.59/
Killed@
>5ppm
/3

.156-.156/3

No Inh/
3
 

No Inh/
3

No Inh/
3

2.5/
Killed@
5ppm
/3

E. typhi-
murium

1.25/Killed@
<5ppm
/3

0.078/
Killed@
>5ppm
/3

No Inh/
3

1.25/
Killed@
>5ppm
/3

5/
Killed @
>5ppm
/3

2.5/
Killed@
5ppm
/3

P. aerug-
inosa

0.078/
Killed@
>5ppm
/3

0.156/
Killed@
0.303ppm

/3

0.130+-
0.037/
Killed@
>5ppm
/3

2.5/
Killed@
>5ppm
/3

2.5/
Killed@
>5ppm/3

1.7+-0.7/
Killed@
5ppm
/3

S. gordonii

0.156/
Killed@
>5ppm
/3

2.5/
Killed@
5ppm

/3

0.012+-
0.005/
Killed@
0.026+-
0.009ppm
/3
 

1.25/
Killed@
>2.5ppm
/3

0.005/
Killed@
0.012+-
0.005ppm
/3

2.5/
Killed@
10ppm
/3

S. aureus

0.313/
Killed@
>5ppm
/3

0.313+-
0.18/
Killed@
0.625ppm
/3

2.5/Killed@
>5ppm
/3

5/Killed@
>5ppm
/3

0.039/
Killed@
>5ppm
/3

5/
Killed@
10ppm
/3

   The average was taken from all data points to obtain the average minimum inhibitory concentration for an antibiotic. Then a standard deviation was determined to give an error. All concentrations were calculated in parts per million (ug/ml). The abbreviation "no inh," stands for "No inhibition of growth." And ">" means that the measurement of the bactericidal concentration was beyond the limits of the test. Information is listed as "MIC (level at which bacteria was inhibited)/MBC (level at which bacteria were killed)/number of tests performed.                                                                                                                                                  

David A. Revelli                                                                          Dr. Ron W. Leavitt, Ph.D. 
Microbiologist                                                                             Professor of Microbiology/Molecular Biology
Brigham Young University                                                         Brigham Young University

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